Journal: Analytical biochemistry

Article Title: Design, synthesis and evaluation of a new fluorescent probe for measuring polymyxin-lipopolysaccharide binding interactions

doi: 10.1016/j.ab.2010.10.033

Figure Lengend Snippet: Fluorometric assay data for the binding affinity of polymyxins to isolated LPS

Article Snippet: Solid lines represent non-linear least squares regression fits as detailed under materials and methods. table ft1 table-wrap mode="anchored" t5 Table1 caption a7 Lipid A K d * ; K i # Binding Affinity (μM) DPmB 3 * [Dansyl- Lys]1polymyxinB3 DPmB * Invitrogen preparation DPmB * in-house preparation PmB # Colistin # PBN # E. coli [ 9 ; 57 ] 0.5±0.1 3.2±0.5 4.6±0.7 0.5±0.1 0.8±0.1 30±4.8 K. pneumoniae ATCC 13883 [ 58 ] 0.6±0.1 2.9±0.6 5.3±0.9 0.5±0.2 0.6±0.1 26±10 S. enteric [ 57 ; 59 ] 0.6±0.1 4.5±0.8 7.0±1.5 0.4±0.1 0.6±0.1 36±8.1 P. aeruginosa [ 42 ] 1.2±0.2 6.8±2.0 8.7±1.8 3.5±0.2 3.8±0.1 48±7.8 Open in a separate window * K d Binding affinity constant determined from direct titration with DPmB or DPmB 3 # K i Binding inhibition constant determined by displacement of DPmB 3 All values are the mean of three independent measurements ± SD Fluorometric assay data for the binding affinity of polymyxins to isolated LPS Binding affinity values for E. coli and A. baumannii whole cells differed markedly from the isolated LPS binding affinity values.

Techniques: Binding Assay, Isolation